• Past exam questions

Course Introduction

Timetable 2003
 

Week	Lecture No	Date	Time	Place	Lect	Topic
1	1	Tues 30/9/03	10	Col 420	EE	GENE EXPRESSION: Introduction to Transcription
	2	Thurs 2/10/03	10	R401	EE	The basal transcription complex:
	3	Fri 3/10/03	10	Col 420	EE	Regulation of transcription; enhancers; use of reporter genes
2	4	Mon 6/10/03	10	Col 420	EE	Expression profiling: Quantitative RT-PCR and other techniques
	5	Tues 7/10/03	10	Col 420	EE	Protein-Nucleic Acid interactions
	6	Thurs 9/10/03	10	R401	EE	Examples of transcriptional regulation I Heat shock; hormonal regulation
	7	Fri 10/10/03	10	R401	EE	Examples of transcriptional regulation II - developmental regulation, cell-specific regulation
3	8	Mon 13/10/03	10	Col 420	EE	Transcription elongation, termination, capping and polyadenylation
	9	Tues 14/10/03	10	Col 420	EE	Nuclear pre-mRNA splicing; mRNA transport
	10	Thurs 16/10/03	10	R401	EE	Translation: General Features
	11	Fri 17/10/03	10	Col 420	EE	Translation in eukaryotes & prokaryotes
4	12	Mon 20/10/03	10	Col 420	EL	Expression systems: His & epitope tags; GFP:SDM; Westerns
	T1	Tues 21/10/03	10	Col 420	EL	Tutorial/CAL exercise
	13	Thurs 23/10/03	10	R 401	EE	GENOMICS Chromosome Structure: DNA replication: general features
	14	Fri 24/10/03	10	Col 420	EE	DNA Replication machinery in prokaryotes and eukaryotes
5	15	Mon 27/10/03	10	Col 420	EE	Chromosome replication; Recombination
	16	Tues 28/10/03	10	Col 420	EE	Genome Structure; Mobile DNA; repeat DNA; DNA fingerprinting
	17	Thurs 30/10/03	10	R 401	EL	Mapping and Sequencing the human genome
	18	Fri 31/10/03	10	Col420	EL	Exploiting the sequence of the human genome
6	19	Mon 3/11/03	10	Col420	EL	Single Gene Disorders: Cloning Disease Genes
	20	Tues 4/11/03	10	Col420	EL	Genetics of Complex Disorders
	21	Thurs 6/11/03	10.	R401	EL	Manipulating Genetic Information: site-directed mutagenesis
	22	Fri 7/11/03	10	Col 420	EL	Applications and Examples: Genetic Testing
7	.	Mon 10/11/03	4pm	R5.25	.	HAND IN ESSAY

Prerequisites: BB211, or similar level classes

Assessment:

• Examination January, 1.5 hours - 80%
• Essay- 20%.

Recommended Reading

• Molecular Cell Biology 4th Edition - Lodish et al (Freeman) 1999 ISBN:0716723808 http://www.whfreeman.com/lodish/
  You can also SEARCH Lodish through ncbi

• Instant notes in Molecular Biology, 2nd Edition, Turner et al (Bios.)
• Human Molecular Genetics Second Edition - Peter Sudbury  (Pearson) ISBN 0-13-042811-6

Class format and delivery:

Class Aims:

• To introduce students to molecular approaches to the study of prokaryotic and eukaryotic genomes (genomics)
• To provide students with a detailed knowledge of the mechanism of gene expression in prokaryotes and eukaryotes.

Learning Outcomes:
On successful completion of this class the student will be able to

1. Understand the structure of chromosomes and how they are replicated.
2. Describe how genomes are mapped and the use that can be made of genome sequence information
3. Understand how disease genes are identified and characterized, and how genetic testing is carried out
4. Understand the molecular mechanisms of transcription, how the expression of genes can be regulated and how this information can be used to overexpress recombinant proteins
5. Understand how eukaryotic genes are processed after transcription and the basic features of translation in eukaryotes and prokaryotes.