BB211:  Cell and Molecular Biology
Dr Eve Lutz
Department of Bioscience
Recombinant DNA technology

Background reading:

References for lectures on recombinant DNA technology please read the following:

Chapter 16 in Klug, WS & Cummings, MR Essentials of Genetics (4th ed).  Prentice Hall (2002) Klug & Cummings 4th ed.

Please see the ReBase website for information regarding Restriction Enzymes: http://rebase.neb.com/rebase/rebhelp.html

For Restriction Mapping, click on this link: Restriction Mapping Page
For animations of various molecular biological techniques, see the following weblink: Biology Animation Library, Dolan DNA Learning Center, Cold Spring Harbor Laboratory 
For Additional Help, click on this link: Helpful Hints

Additional information on Recombinant DNA techniques can be obtained from the following sources:

  1. Chapter 6, sections 6 & 7; and chapter 10  Hartl, D & Jones E Essential Genetics, 2nd ed.
  2. Chapter 8, pp 491-513 in Alberts, Johnson, Lewis, Raff, Roberts & Walter Molecular Biology of the Cell (4th ed).
  3. Chapter 7 in Lodish et al. Molecular Cell Biology (4th ed). Freeman Press (2000) Lodish et al, 4th edition
  4. Sections G-K in Turner et al, (2nd ed.) Instant Notes in Molecular Biology

Please note, these web notes are meant for background reading and are not intended for handouts to the lecture. Lecture notes will be handed out before each lecture.



Lecture 1: Introduction to Recombinant DNA technology. Restriction enzymes & gel electrophoresis
Lecture 2: Southern, Northern Analysis
Lecture 3: Plasmids, cloning
Lecture 4: Making & Screening Libraries
Lecture 5: DNA sequencing
Lecture 6: Polymerase chain reaction & applications

webpage last updated 21/2/03