BB211:
Cell and Molecular Biology
Dr Eve Lutz
Department of Bioscience
Recombinant DNA technology
Background reading:
References for lectures on
recombinant DNA technology please read the following:
Chapter 16 in Klug, WS & Cummings, MR Essentials
of Genetics (4th ed). Prentice Hall (2002) Klug & Cummings 4th ed.
Please see the ReBase website
for information regarding Restriction Enzymes: http://rebase.neb.com/rebase/rebhelp.html
For Restriction Mapping, click
on this link: Restriction
Mapping Page
For animations of various molecular
biological techniques, see the following weblink: Biology
Animation Library, Dolan DNA Learning Center, Cold Spring Harbor Laboratory
For Additional Help, click on
this link: Helpful
Hints
Additional information on Recombinant
DNA techniques can be obtained from the following sources:
- Chapter 6, sections 6 & 7; and
chapter 10 Hartl,
D & Jones E Essential Genetics,
2nd ed.
- Chapter 8, pp 491-513
in Alberts, Johnson, Lewis, Raff, Roberts & Walter Molecular Biology of the Cell (4th ed).
- Chapter 7 in Lodish et al. Molecular
Cell Biology (4th ed). Freeman Press
(2000) Lodish et al, 4th edition
- Sections G-K in Turner et al, (2nd
ed.) Instant Notes in Molecular Biology
Please note, these web notes
are meant for background reading and are not intended for handouts to the
lecture. Lecture notes will be handed out before each lecture.
Lecture
1: Introduction to Recombinant DNA technology. Restriction enzymes &
gel electrophoresis
Lecture
2: Southern, Northern Analysis
Lecture
3: Plasmids, cloning
Lecture
4: Making & Screening Libraries
Lecture
5: DNA sequencing
Lecture
6: Polymerase chain reaction & applications
webpage last updated 21/2/03